The website "wirelessprobe.org" is a collaborative platform that serves as a hub for sharing design files, software, and relevant information related to wireless optogenetic probes. We aim to facilitate the dissemination of this easy-to-use optogenetic tool to the wider neuroscience community and foster a vibrant user community that drives its continuous advancement and contributes to the project.
Although the wireless optogenetic probe system described here is not a readily available commercial solution, we have placed significant emphasis on ensuring its accessibility to the neuroscience laboratory. The device system is designed for ease of use, enabling simple hands-on assembly and implementation.
We will consistently update the wiki site with new developments and resources as we make progress.
Design Overview
The wireless optogenetic probes available in this wiki site have the following designs and wireless operation capabilities:
Unilateral and bilateral probes with varying length, gap, and wavelength (details can be found in this table)
Wireless operation using smartphone control
[Wireless optogenetic probes with unilateral and bilateral design available on this wiki]
Bluetooth Control of Wireless Optogenetic Devices,
as published in Nature Biomedical Engineering
Wireless Optogenetic Control of Locomotor Activity in Mice
Developers of the wireless optogenetic probes have successfully demonstrated and utilized various versions of these probes in a range of behavior neuroscience studies, as documented in the following publications:
Jeon et al., “Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female mice”, Journal of Neuroscience 43, 1555-1571 (2023) → Link
Parker et al., “Customizable, wireless and implantable neural probe design and fabrication via 3D printing”, Nature Protocols 18, 3-21 (2023) → Link
Qazi et al., “Scalable and modular wireless-network infrastructure for large-scale behavioural neuroscience”, Nature Biomedical Engineering 6, 771-786 (2022) → Link
Kim et al., “Soft subdermal implant capable of wireless battery charging and programmable controls for applications in optogenetics”, Nature Communications 12, 535 (2021) → Link
Lee et al., “Rapidly-customizable, scalable 3D-printed wireless optogenetic probes for versatile applications in neuroscience”, Advanced Functional Materials 30, 2004285 (2020) → Link
Byun et al., “Mechanically transformative electronics, sensors, and implantable devices”, Science Advances 5, eaay0418 (2019) → Link
Qazi et al., “Wireless optofluidic brain probes for chronic neuropharmacology and photostimulation”, Nature Biomedical Engineering 3, 655-669 (2019) → Link
We encourage the probe users to provide feedback and comments to contribute to future improvements and developments.
If you require custom designs and advanced wireless operations, such as closed-loop control, please reach out to the developers for opportunities to collaborate.
Guide and Tutorial
To build wireless optogenetic probes, you will need the following components:
Component list and procurement (for microLED probe and wireless control module)
For software codes required for the project, you can download them from the following link:
To learn how to construct wireless optogenetic probes, you can refer to the tutorial available here:
For additional information on wireless optogenetic probes, please refer to the following resources:
Surgical procedure (Please refer to “Implantation of 3D-POPs into targeted brain structure” (p.13-16) in Procedure 3, Steps 1-15)
Optogenetic behavior assay guide (Please refer to “Preparation of the behavior assay for optogenetic stimulation” (p.16) in Procedure 3, Steps 16-17 and “Performing the in vivo optogenetic behavioral assay” (p.16-17) in Procedure 3, Steps 18-23)
Discussion & Question Board
Google Group Discussion Board: Use this board to share any queries or initiate discussions.